Dead Cas Systems: Types, Principles, and Applications.
Int J Mol Sci
; 20(23)2019 Nov 30.
Article
em En
| MEDLINE
| ID: mdl-31801211
ABSTRACT
The gene editing tool CRISPR-Cas has become the foundation for developing numerous molecular systems used in research and, increasingly, in medical practice. In particular, Cas proteins devoid of nucleolytic activity (dead Cas proteins; dCas) can be used to deliver functional cargo to programmed sites in the genome. In this review, we describe current CRISPR systems used for developing different dCas-based molecular approaches and summarize their most significant applications. We conclude with comments on the state-of-art in the CRISPR field and future directions.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Doenças Transmissíveis
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Sistemas CRISPR-Cas
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Edição de Genes
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Proteína 9 Associada à CRISPR
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Doenças Genéticas Inatas
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Inflamação
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Neoplasias
Limite:
Humans
Idioma:
En
Revista:
Int J Mol Sci
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Federação Russa