Current advances in microcell-mediated chromosome transfer technology and its applications.
Exp Cell Res
; 390(1): 111915, 2020 05 01.
Article
em En
| MEDLINE
| ID: mdl-32092294
ABSTRACT
Chromosomes and chromosomal gene delivery vectors, human/mouse artificial chromosomes (HACs/MACs), can introduce megabase-order DNA sequences into target cells and are used for applications including gene mapping, gene expression control, gene/cell therapy, and the development of humanized animals and animal models of human disease. Microcell-mediated chromosome transfer (MMCT), which enables chromosome transfer from donor cells to target cells, is a key technology for these applications. In this review, we summarize the principles of gene transfer with HACs/MACs; their engineering, characteristics, and utility; and recent advances in the chromosome transfer technology.
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Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Engenharia Genética
/
Técnicas de Transferência de Genes
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Cromossomos Artificiais de Mamíferos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Exp Cell Res
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Japão