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IL-17A inhibitions of indole alkaloids from traditional Chinese medicine Qing Dai.
Lee, Chia-Lin; Wang, Chien-Ming; Kuo, Yueh-Hsiung; Yen, Hung-Rong; Song, Ying-Chyi; Chou, Yu-Lun; Chen, Chao-Jung.
Afiliação
  • Lee CL; Department of Cosmeceutics, China Medical University, Taichung, 40402, Taiwan; Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung, 40447, Taiwan; Chinese Medicine Research Center, China Medical University, Taichung, 40402, Taiwan. Electronic address: chlile
  • Wang CM; Chinese Medicine Research Center, China Medical University, Taichung, 40402, Taiwan. Electronic address: magic2ming@yahoo.com.tw.
  • Kuo YH; Chinese Medicine Research Center, China Medical University, Taichung, 40402, Taiwan; Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, 40402, Taiwan; Department of Biotechnology, Asia University, Taichung, 41354, Taiwan. Electronic addr
  • Yen HR; Chinese Medicine Research Center, China Medical University, Taichung, 40402, Taiwan; Graduate Institute of Chinese Medicine, School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung, 40402, Taiwan; Department of Chinese Medicine, China Medical University Hospital,
  • Song YC; Chinese Medicine Research Center, China Medical University, Taichung, 40402, Taiwan; Graduate Institute of Integrated Medicine, China Medical University, Taichung, 40402, Taiwan. Electronic address: songyingchyi@gmail.com.
  • Chou YL; Chinese Medicine Research Center, China Medical University, Taichung, 40402, Taiwan; Research Center for Traditional Chinese Medicine, Department of Medical Research, China Medical University Hospital, Taichung, 40447, Taiwan. Electronic address: lulume770825@gmail.com.
  • Chen CJ; Graduate Institute of Integrated Medicine, China Medical University, Taichung, 40402, Taiwan; Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, 40447, Taiwan. Electronic address: ironmanchen@yahoo.com.tw.
J Ethnopharmacol ; 255: 112772, 2020 Jun 12.
Article em En | MEDLINE | ID: mdl-32194230
ETHNOPHARMACOLOGICAL RELEVANCE: Qing Dai, a famous traditional Chinese medicine (TCM), is prepared by a traditional fermentation process with the aerial part of Strobilanthes cusia. Currently, this TCM could treat various clinical inflammatory diseases, such as ulcerative colitis and psoriasis, however, the bioactive components of Qing Dai are unknown clearly. AIM OF THE STUDY: To isolate and identify the anti-IL-17A components of Qing Dai. MATERIALS AND METHODS: Silica, RP-18 gels, and size exclusion resin were used for column chromatography to isolate the pure compounds. The structures of isolates were elucidated by NMR, MS, UV, IR spectra, and optical rotation. IL-17A protein and gene expressions were also evaluated in the Th17 cell model and luciferase reporter assay, respectively. RESULTS: Two indole alkaloids, including one new indigodole D and cephalandole B, were isolated from Qing Dai. Indigodole D could inhibit IL-17A protein production during the Th17 polarization (EC50: 2.16 µg/mL) or after the polarization (EC50: 5.99 µg/mL) without cytotoxicity toward Th17 cells. Cephalandole B did not inhibit the IL-17A protein secretion. Nevertheless, both isolates notably inhibited IL-17A gene expression, especially cephalandole B, in a dose-dependent manner in Jukat cells with IL-17A luciferase reporter. CONCLUSIONS: Indole alkaloids, indigodoles A, C, D, tryptanthrin, and indirubin could contribute to anti-IL 17A properties of Qing Dai. The possible biogenetic mechanisms of above-mentioned indoles were also speculated in this investigation for further promising anti-IL-17 lead drugs development.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Medicamentos de Ervas Chinesas / Interleucina-17 / Alcaloides Indólicos / Células Th17 / Anti-Inflamatórios Limite: Animals / Humans Idioma: En Revista: J Ethnopharmacol Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Medicamentos de Ervas Chinesas / Interleucina-17 / Alcaloides Indólicos / Células Th17 / Anti-Inflamatórios Limite: Animals / Humans Idioma: En Revista: J Ethnopharmacol Ano de publicação: 2020 Tipo de documento: Article