DNA extraction from sternum bone for identification of a saponified body: use of a modified protocol.
Anthropol Anz
; 77(3): 235-242, 2020 Aug 12.
Article
em En
| MEDLINE
| ID: mdl-32211746
ABSTRACT
This paper discusses our approach and results obtained when attempting to identify a saponified human body recovered from the sea, without arms and legs. Bones, especially the long ones, are the only sources of DNA available in several cases involving unidentified bodies in advanced state of putrefaction. In this case, since the body was found without limbs, attempts were made to extract DNA from the sternum bone. The DNA was extracted using a modified version of the NucleoSpin® DNA Trace Kit (Macherey Nagel™) protocol and an STR analysis was performed. Thanks to this modified protocol a complete DNA profile was obtained from the sternum bone, while only partial results were obtained from blood and teeth. The DNA profile obtained from the sternum was compared with the DNA of the putative son searching for a genetic match. Five incompatibilities were detected so it was possible to exclude the kinship. In conclusion this could be a useful technique in personal identification through DNA analysis in case of poor quality and quantity of bone.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Dente
/
DNA
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Impressões Digitais de DNA
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Anthropol Anz
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Itália