An efficient Screening System in Yeast to Select a Hyperactive <i>piggyBac</i> Transposase for Mammalian Applications.

Wen, Wen; Song, Shanshan; Han, Yuchun; Chen, Haibin; Liu, Xiangzhen; Qian, Qijun

An efficient Screening System in Yeast to Select a Hyperactive piggyBac Transposase for Mammalian Applications.

Wen, Wen; Song, Shanshan; Han, Yuchun; Chen, Haibin; Liu, Xiangzhen; Qian, Qijun.

Afiliação

Wen W; Shanghai Cell Therapy Research Institute, Shanghai Cell Therapy Group, Shanghai 201805, China.
Song S; Shanghai Cell Therapy Research Institute, Shanghai Cell Therapy Group, Shanghai 201805, China.
Han Y; Shanghai Cell Therapy Research Institute, Shanghai Cell Therapy Group, Shanghai 201805, China.
Chen H; Shanghai Cell Therapy Research Institute, Shanghai Cell Therapy Group, Shanghai 201805, China.
Liu X; Shanghai Cell Therapy Research Institute, Shanghai Cell Therapy Group, Shanghai 201805, China.
Qian Q; Shanghai Cell Therapy Research Institute, Shanghai Cell Therapy Group, Shanghai 201805, China.

Int J Mol Sci ; 21(9)2020 Apr 26.

Article em En | MEDLINE | ID: mdl-32357554

Assuntos

Plasmídeos/genética; Saccharomyces cerevisiae/enzimologia; Transposases/genética; Animais; Células CHO; Cricetulus; Elementos de DNA Transponíveis; Edição de Genes; Ensaios de Triagem em Larga Escala; Mutação; Saccharomyces cerevisiae/genética; Transposases/metabolismo

Palavras-chave

efficient screening system; non-viral transgenic vectors; piggyBac transposase; transposition efficiency; yeast

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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Plasmídeos / Saccharomyces cerevisiae / Transposases Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Animals Idioma: En Revista: Int J Mol Sci Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China

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