Assessing the Use of PCR To Screen for Shedding of Salmonella enterica in Infected Humans.
J Clin Microbiol
; 58(7)2020 06 24.
Article
em En
| MEDLINE
| ID: mdl-32376667
ABSTRACT
Recovery from enteric bacterial illness often includes a phase of organismal shedding over a period of days to months. The monitoring of this process through laboratory testing forms the foundation of public health action to prevent further transmission. Regulations in most jurisdictions in the United States exclude individuals who continue to shed certain organisms from sensitive occupations and situations, such as food handling, providing direct patient care, or attending day care. The burden that this creates for recovering patients and their families/coworkers is great, so any effort to provide efficiency to the testing process would be of significant benefit. We sought to assess the ability of PCR for the detection of Salmonella enterica shedding and to compare that ability to culture-based testing. PCR would be faster than culture and would allow results to be generated more quickly. Herein, we show data that indicate that, while PCR and culture testing agree in the majority of cases, there are incidents of discordance between the two tests, whereupon PCR shows positive results when culture indicates lack of detectable viable organisms. Using culture-based testing as the standard, the negative predictive value of PCR was found to be 100%, while the positive predictive value was 79%. The nature of this discordance is briefly investigated. We found that it is possible that PCR may not only detect nonviable organisms in stool but also viable organisms that remain undetectable by standard culture methods.
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Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Salmonella enterica
Limite:
Humans
Idioma:
En
Revista:
J Clin Microbiol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos