Effective on-line high-speed shear dispersing emulsifier technique coupled with high-performance countercurrent chromatography method for simultaneous extraction and isolation of carotenoids from Lycium barbarum L. fruits.
J Sep Sci
; 43(14): 2949-2958, 2020 Jul.
Article
em En
| MEDLINE
| ID: mdl-32384220
ABSTRACT
An efficient combination strategy based on high-speed shear dispersing emulsifier technique and high-performance countercurrent chromatography was developed for on-line extraction and isolation of carotenoids from the fruits of Lycium barbarum. In this work, the high-speed shear dispersing emulsifier technique has been employed to extract crude extracts using the upper phase of high-performance countercurrent chromatography solvent system composed of n-hexane-dichloromethane-acetonitrile (1046.5, v/v) as the extraction solvent. At the separation stage, the high-performance counter-current chromatography process adopts elution-extrusion mode and the upper phase of the solvent system as stationary phase (reverse-phase mode). As a result, three compounds including zeaxanthin, zeaxanthin monopalmitate, and zeaxanthin dipalmitate with purities of 89, 90, and 93% were successfully obtained in one extraction-separation operation within 120 min. The targeted compounds were analyzed and identified by high-performance liquid chromatography, mass spectrometry, and NMR spectroscopy. The results indicated that the present on-line combination method could serve as a simple, rapid, and effective way to achieve weak polar and unstable compounds from natural products.
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Bases de dados:
MEDLINE
Assunto principal:
Carotenoides
/
Lycium
Idioma:
En
Revista:
J Sep Sci
Ano de publicação:
2020
Tipo de documento:
Article