Prenatal ethanol exposure-induced hypothalamic an imbalance of glutamatergic/GABAergic projections and low functional expression in male offspring rats.
Food Chem Toxicol
; 141: 111419, 2020 Jul.
Article
em En
| MEDLINE
| ID: mdl-32437893
ABSTRACT
This study was designed to demonstrate that prenatal ethanol exposure (PEE) can induce low functional expression of the hypothalamus in male offspring rats and explore the underlying mechanism. Pregnant rats were administered 4 g/kg ethanol or normal saline by oral gavage each day from gestational day (GD) 9 to GD20. Male GD20 foetuses and postnatal day 120 adult offspring rats were sacrificed under anaesthesia. Hypothalamic cells from male GD20~postnatal day (PD) 7 rats were treated with different doses of corticosterone and the glucocorticoid receptor (GR) antagonist mifepristone for 5 days. In this study, we found that PEE-induced overexposure of maternal glucocorticoids enhanced the expression of L-glutamic acid decarboxylase (GAD) 67 in the hypothalamic paraventricular nucleus (PVN) by activating the glucocorticoid metabolic activation system, further inducing the conversion of glutamate to L-gamma-aminobutyric acid (GABA) and developmental imbalance of glutamatergic/GABAergic projections to the PVN. The imbalance change was maintained until after birth, resulting in the inhibition of parvocellular neurons and low functional expression of the hypothalamus in PEE offspring rats. Our study indicated that low functional expression of the hypothalamus in male PEE offspring rats was associated with developmental programming of an imbalance of glutamatergic/GABAergic projections to the PVN.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Núcleo Hipotalâmico Paraventricular
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Efeitos Tardios da Exposição Pré-Natal
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Ácido Glutâmico
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Etanol
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Ácido gama-Aminobutírico
Limite:
Animals
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Pregnancy
Idioma:
En
Revista:
Food Chem Toxicol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China