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Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936.
Toombs, Jamie; Panther, Lindsay; Ornelas, Loren; Liu, Chunyan; Gomez, Emilda; Martín-Ibáñez, Raquel; Cox, Simon R; Ritchie, Stuart J; Harris, Sarah E; Taylor, Adele; Redmond, Paul; Russ, Tom C; Murphy, Lee; Cooper, James D; Burr, Karen; Selvaraj, Bhuvaneish T; Browne, Cathy; Svendsen, Clive N; Cowley, Sally A; Deary, Ian J; Chandran, Siddharthan; Spires-Jones, Tara L; Sareen, Dhruv.
Afiliação
  • Toombs J; Centre for Discovery Brain Sciences, UK Dementia Research Institute, The University of Edinburgh, UK.
  • Panther L; iPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA.
  • Ornelas L; iPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA.
  • Liu C; iPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA.
  • Gomez E; iPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA.
  • Martín-Ibáñez R; Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA.
  • Cox SR; Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK.
  • Ritchie SJ; Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK.
  • Harris SE; Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK.
  • Taylor A; Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK.
  • Redmond P; Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK.
  • Russ TC; Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK; Alzheimer Scotland Dementia Research Centre, University of Edinburgh, Edinburgh, UK.
  • Murphy L; Edinburgh Clinical Research Facility, University of Edinburgh, Edinburgh, UK.
  • Cooper JD; Dementia Research Institute at the University of Edinburgh, UK; Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, Scotland, UK.
  • Burr K; Dementia Research Institute at the University of Edinburgh, UK; Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, Scotland, UK.
  • Selvaraj BT; Dementia Research Institute at the University of Edinburgh, UK; Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, Scotland, UK.
  • Browne C; James Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
  • Svendsen CN; Board of Governors-Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA.
  • Cowley SA; James Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, Oxford, UK; Oxford Parkinson's Disease Centre, Oxford, UK.
  • Deary IJ; Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK.
  • Chandran S; Dementia Research Institute at the University of Edinburgh, UK; Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, Scotland, UK.
  • Spires-Jones TL; Centre for Discovery Brain Sciences, UK Dementia Research Institute, The University of Edinburgh, UK. Electronic address: tara.spires-jones@ed.ac.uk.
  • Sareen D; iPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA; Board of Governors-Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048,
Stem Cell Res ; 46: 101851, 2020 07.
Article em En | MEDLINE | ID: mdl-32450543
ABSTRACT
Cognitive decline is among the most feared aspects of ageing. We have generated induced pluripotent stem cells (iPSCs) from 24 people from the Lothian Birth Cohort 1936, whose cognitive ability was tested in childhood and in older age. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating oriP/EBNA1 backbone plasmids expressing six iPSC reprogramming factors (OCT3/4 (POU5F1), SOX2, KLF4, L-Myc, shp53, Lin28, SV40LT). All lines demonstrated STR matched karyotype and pluripotency was validated by multiple methods. These iPSC lines are a valuable resource to study molecular mechanisms underlying individual differences in cognitive ageing and resilience to age-related neurodegenerative diseases.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes Induzidas Idioma: En Revista: Stem Cell Res Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes Induzidas Idioma: En Revista: Stem Cell Res Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Reino Unido