Your browser doesn't support javascript.
loading
Competition between maturation and degradation drives human snRNA 3' end quality control.
Lardelli, Rea M; Lykke-Andersen, Jens.
Afiliação
  • Lardelli RM; Division of Biological Sciences, University of California San Diego, La Jolla, California 92093, USA.
  • Lykke-Andersen J; Division of Biological Sciences, University of California San Diego, La Jolla, California 92093, USA.
Genes Dev ; 34(13-14): 989-1001, 2020 07 01.
Article em En | MEDLINE | ID: mdl-32499401
Polymerases and exonucleases act on 3' ends of nascent RNAs to promote their maturation or degradation but how the balance between these activities is controlled to dictate the fates of cellular RNAs remains poorly understood. Here, we identify a central role for the human DEDD deadenylase TOE1 in distinguishing the fates of small nuclear (sn)RNAs of the spliceosome from unstable genome-encoded snRNA variants. We found that TOE1 promotes maturation of all regular RNA polymerase II transcribed snRNAs of the major and minor spliceosomes by removing posttranscriptional oligo(A) tails, trimming 3' ends, and preventing nuclear exosome targeting. In contrast, TOE1 promotes little to no maturation of tested U1 variant snRNAs, which are instead targeted by the nuclear exosome. These observations suggest that TOE1 is positioned at the center of a 3' end quality control pathway that selectively promotes maturation and stability of regular snRNAs while leaving snRNA variants unprocessed and exposed to degradation in what could be a widespread mechanism of RNA quality control given the large number of noncoding RNAs processed by DEDD deadenylases.
Assuntos
Palavras-chave

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Proteínas Nucleares / RNA Nuclear Pequeno / Estabilidade de RNA / Processamento de Terminações 3' de RNA Limite: Humans Idioma: En Revista: Genes Dev Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Proteínas Nucleares / RNA Nuclear Pequeno / Estabilidade de RNA / Processamento de Terminações 3' de RNA Limite: Humans Idioma: En Revista: Genes Dev Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos