Production of Intrinsically Disordered Proteins for Biophysical Studies: Tips and Tricks.

Pedersen, Christian Parsbæk; Seiffert, Pernille; Brakti, Inna; Bugge, Katrine

Pedersen, Christian Parsbæk; Seiffert, Pernille; Brakti, Inna; Bugge, Katrine.

Afiliação

Pedersen CP; Structural Biology and NMR Laboratory and the Linderstrøm-Lang Centre for Protein Science, Department of Biology, University of Copenhagen, Copenhagen N, Denmark.
Seiffert P; Structural Biology and NMR Laboratory and the Linderstrøm-Lang Centre for Protein Science, Department of Biology, University of Copenhagen, Copenhagen N, Denmark.
Brakti I; Structural Biology and NMR Laboratory and the Linderstrøm-Lang Centre for Protein Science, Department of Biology, University of Copenhagen, Copenhagen N, Denmark.
Bugge K; Structural Biology and NMR Laboratory and the Linderstrøm-Lang Centre for Protein Science, Department of Biology, University of Copenhagen, Copenhagen N, Denmark. katrine.bugge@bio.ku.dk.

Methods Mol Biol ; 2141: 195-209, 2020.

Article em En | MEDLINE | ID: mdl-32696358

Assuntos

Biofísica/métodos; Proteínas Intrinsicamente Desordenadas/biossíntese; Precipitação Química; Cromatografia de Fase Reversa; Temperatura Alta; Proteínas Intrinsicamente Desordenadas/isolamento & purificação; Ponto Isoelétrico; Desnaturação Proteica

Palavras-chave

Heat treatment; IDP; Intrinsically disordered proteins; Ion-exchange chromatography; Isoelectric precipitation; Protein purification; Purification optimization; Reversed-phase chromatography; Troubleshooting

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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Biofísica / Proteínas Intrinsicamente Desordenadas Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Dinamarca

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