Optimized Protocol for the In Situ Derivatization of Glutathione with <i>N</i>-Ethylmaleimide in Cultured Cells and the Simultaneous Determination of Glutathione/Glutathione Disulfide Ratio by HPLC-UV-QTOF-MS.

Sun, Xueni; Berger, Raffaela S; Heinrich, Paul; Marchiq, Ibtissam; Pouyssegur, Jacques; Renner, Kathrin; Oefner, Peter J; Dettmer, Katja

Optimized Protocol for the In Situ Derivatization of Glutathione with N-Ethylmaleimide in Cultured Cells and the Simultaneous Determination of Glutathione/Glutathione Disulfide Ratio by HPLC-UV-QTOF-MS.

Sun, Xueni; Berger, Raffaela S; Heinrich, Paul; Marchiq, Ibtissam; Pouyssegur, Jacques; Renner, Kathrin; Oefner, Peter J; Dettmer, Katja.

Afiliação

Sun X; Institute of Functional Genomics, University of Regensburg, Am BioPark 9, 93053 Regensburg, Germany.
Berger RS; Institute of Functional Genomics, University of Regensburg, Am BioPark 9, 93053 Regensburg, Germany.
Heinrich P; Institute of Functional Genomics, University of Regensburg, Am BioPark 9, 93053 Regensburg, Germany.
Marchiq I; University Côte d'Azur (IRCAN), CNRS-INSERM, Centre A. Lacassagne, 06189 Nice, France.
Pouyssegur J; University Côte d'Azur (IRCAN), CNRS-INSERM, Centre A. Lacassagne, 06189 Nice, France.
Renner K; Department of Medical Biology, Centre Scientifique de Monaco, CSM, 98000 Monaco, Monaco.
Oefner PJ; Department of Internal Medicine III, University Hospital Regensburg, 93042 Regensburg, Germany.
Dettmer K; Institute of Functional Genomics, University of Regensburg, Am BioPark 9, 93053 Regensburg, Germany.

Metabolites ; 10(7)2020 Jul 17.

Article em En | MEDLINE | ID: mdl-32709039

Palavras-chave

UV detection; cell culture; glutathione; glutathione disulfide; liquid chromatography; mass spectrometry

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Texto completo: 1 Bases de dados: MEDLINE Idioma: En Revista: Metabolites Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Alemanha