Isolation and plasmid characterisation of Salmonella enterica serovar Albany harbouring mcr-5 from retail chicken meat in Japan.
FEMS Microbiol Lett
; 367(15)2020 08 01.
Article
em En
| MEDLINE
| ID: mdl-32756977
ABSTRACT
The emergence of plasmid-mediated colistin resistance genes (mcr), which is occurring in numerous countries, is a worldwide concern, primarily because colistin is a last-resort antibiotic. Compared to E. coli, prevalence of mcr genes in Salmonella is unclear in Japan. Here we screened for mcr-1-5 genes in our collection of Salmonella strains isolated from retail meat products collected in Japan from 2012 through 2016. We found that Salmonella Albany strain 27A-368 encodes mcr-5 and that mcr genes were undetectable among the remaining 202 isolates. The resistance plasmid p27A-368 was transferred by conjugation to S. Infantis and was stably retained as a transconjugant. Whole-genome sequencing revealed that mcr-5 resided on a 115 kb plasmid (p27A-368). The plasmid backbone of p27A-368 is more similar to that of pCOV27, an ESBL-encoding plasmid recovered from avian pathogenic E. coli, rather than pSE13-SA01718 of S. Paratyphi B that encodes mcr-5. Further, mcr-5 is located on a transposon, and its sequence is similar to that of pSE13-SA01718. A phylogenetic tree based on single nucleotide variants implies a relationship between 27A-368 and S. Albany isolated in Southeast Asian countries.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Salmonella enterica
/
Microbiologia de Alimentos
/
Carne
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Japão