SARS-CoV-2 Spike Protein Interacts with Multiple Innate Immune Receptors.
bioRxiv
; 2020 Jul 30.
Article
em En
| MEDLINE
| ID: mdl-32766577
ABSTRACT
The spike (S) glycoprotein in the envelope of SARS-CoV-2 is densely glycosylated but the functions of its glycosylation are unknown. Here we demonstrate that S is recognized in a glycan-dependent manner by multiple innate immune receptors including the mannose receptor MR/CD206, DC-SIGN/CD209, L-SIGN/CD209L, and MGL/CLEC10A/CD301. Single-cell RNA sequencing analyses indicate that such receptors are highly expressed in innate immune cells in tissues susceptible to SARS-CoV-2 infection. Binding of the above receptors to S is characterized by affinities in the picomolar range and consistent with S glycosylation analysis demonstrating a variety of N- and O-glycans as receptor ligands. These results indicate multiple routes for SARS-CoV-2 to interact with human cells and suggest alternative strategies for therapeutic intervention.
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Bases de dados:
MEDLINE
Idioma:
En
Revista:
BioRxiv
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos