Infection-Induced Porcine Ex Vivo Corneal Wound Model to Study the Efficacy of Herpes Simplex Virus-1 Entry and Replication Inhibitors.
Methods Mol Biol
; 2193: 183-196, 2021.
Article
em En
| MEDLINE
| ID: mdl-32808270
ABSTRACT
Corneal infections by viruses and bacteria can result in ocular surface defects, ulcers, or wounds. Herpes simplex virus type-1 (HSV-1) is a human virus with global seroprevalence in the range of 60-90%. While the virus more commonly causes mucocutaneous lesions including ulcers on the face and mouth, it is also a leading cause of infection-associated blindness. In this chapter, we discuss an in-depth protocol required to evaluate corneal damage due to HSV-1 infection using porcine models of ex vivo infection. Our methods can be adapted to study similar infections caused by other viruses and bacteria.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Ceratite Herpética
/
Simplexvirus
/
Técnicas de Cultura de Tecidos
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Modelos Animais de Doenças
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Lesões da Córnea
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos