Pneumatically Sprayed Gold Nanoparticles for Mass Spectrometry Imaging of Neurotransmitters.
J Am Soc Mass Spectrom
; 31(12): 2452-2461, 2020 Dec 02.
Article
em En
| MEDLINE
| ID: mdl-32841002
ABSTRACT
Using citrate-capped gold nanoparticles (AuNPs) for laser desorption ionization mass spectrometry (LDI-MS) is an approach that has demonstrated broad applicability to ionization of different classes of molecules. Here, we show a simple AuNP-based approach for the ionization of neurotransmitters. Specifically, the detection of acetylcholine, dopamine, epinephrine, glutamine, 4-aminobutyric acid, norepinephrine, octopamine, and serotonin was achieved at physiologically relevant concentrations in serum and homogenized tissue. Additionally, pneumatic spraying of AuNPs onto tissue sections facilitated mass spectrometry imaging (MSI) of rabbit brain tissue sections, zebrafish embryos, and neuroblastoma cells for several neurotransmitters simultaneously using this quick and simple sample preparation. AuNP LDI-MS achieved mapping of neurotransmitters in fine structures of zebrafish embryos and neuroblastoma cells at a lateral spatial resolution of 5 µm. The use of AuNPs to ionize small aminergic neurotransmitters in situ provides a fast, high-spatial resolution method for simultaneous detection of a class of molecules that typically evade comprehensive detection with traditional matrixes.
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Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Neurotransmissores
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Am Soc Mass Spectrom
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos