Neural Regenerative Potential of Stem Cells Derived from the Tooth Apical Papilla.
Stem Cells Dev
; 29(23): 1479-1496, 2020 12 01.
Article
em En
| MEDLINE
| ID: mdl-32988295
ABSTRACT
The regenerative effects of stem cells derived from dental tissues have been previously investigated. This study assessed the potential of human tooth stem cells from apical papilla (SCAP) on nerve regeneration. The SCAP collected from nine individuals were characterized and polarized by exposure to interferon-γ (IFN-γ). IFN-γ increased kynurenine and interleukin-6 (IL-6) production by SCAP, without affecting the cell viability. IFN-γ-primed SCAP exhibited a decrease of brain-derived neurotrophic factor (BDNF) mRNA levels, followed by an upregulation of glial cell-derived neurotrophic factor mRNA. Ex vivo, the co-culture of SCAP with neurons isolated from the rat dorsal root ganglion induced neurite outgrowth, accompanied by increased BDNF secretion, irrespective of IFN-γ priming. In vivo, the local application of SCAP reduced the mechanical and thermal hypersensitivity in Wistar rats that had been submitted to sciatic chronic constriction injury. The SCAP also reduced the pain scores, according to the evaluation of the Grimace scale, partially restoring the myelin damage and BDNF immunopositivity secondary to nerve lesion. Altogether, our results provide novel evidence about the regenerative effects of human SCAP, indicating their potential to handle nerve injury-related complications.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Papila Dentária
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Transplante de Células-Tronco Mesenquimais
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Células-Tronco Mesenquimais
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Regeneração Nervosa
Tipo de estudo:
Prognostic_studies
Limite:
Adolescent
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Adult
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Animals
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Humans
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Male
Idioma:
En
Revista:
Stem Cells Dev
Assunto da revista:
HEMATOLOGIA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Brasil