An optimized acetylcholine sensor for monitoring in vivo cholinergic activity.
Nat Methods
; 17(11): 1139-1146, 2020 11.
Article
em En
| MEDLINE
| ID: mdl-32989318
ABSTRACT
The ability to directly measure acetylcholine (ACh) release is an essential step toward understanding its physiological function. Here we optimized the GRABACh (GPCR-activation-based ACh) sensor to achieve substantially improved sensitivity in ACh detection, as well as reduced downstream coupling to intracellular pathways. The improved version of the ACh sensor retains the subsecond response kinetics, physiologically relevant affinity and precise molecular specificity for ACh of its predecessor. Using this sensor, we revealed compartmental ACh signals in the olfactory center of transgenic flies in response to external stimuli including odor and body shock. Using fiber photometry recording and two-photon imaging, our ACh sensor also enabled sensitive detection of single-trial ACh dynamics in multiple brain regions in mice performing a variety of behaviors.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Encéfalo
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Técnicas Biossensoriais
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Acetilcolina
Limite:
Animals
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Humans
Idioma:
En
Revista:
Nat Methods
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China