Seminal cell-free DNA and sperm characteristic's: An added biomarker for male infertility investigation.
Andrologia
; 53(1): e13822, 2021 Feb.
Article
em En
| MEDLINE
| ID: mdl-33040391
ABSTRACT
Cell-free DNA (Cf-DNA) fragments may constitute an easy-to-measure molecular tool for guiding the choice of care provided to infertile couples who benefit assisted reproductive technology (ART) programmes. Data on Cf-DNA levels in the seminal plasma of men with sperm alterations are scarce. The objective of the present study was to quantify the presence of Cf-DNA in semen by using a quantitative real-time PCR. We compared men with abnormal sperm characteristics (n = 21) with normospermic controls (n = 21). The PCR assay evidenced significantly higher mean Cf-DNA levels in patients with sperm abnormalities than in controls (2.09 versus 1.18 µg/ml, respectively; p = .0003). The Cf-DNA levels were notably higher in men with azoospermia (3.65 µg/ml, versus 1.34 µg/ml in matched controls; p = .03) and men with teratozoospermia (1.80 µg/ml, versus 1.29 µg/ml in matched controls; p = .008). Our data report a significant association between elevated Cf-DNA levels and sperm abnormalities. These results may open up new diagnostic and prognostic perspectives in male infertility.
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Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Ácidos Nucleicos Livres
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Infertilidade Masculina
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Limite:
Humans
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Male
Idioma:
En
Revista:
Andrologia
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
França