Your browser doesn't support javascript.
loading
Glucosinolate Content in Brassica Genetic Resources and Their Distribution Pattern within and between Inner, Middle, and Outer Leaves.
Rhee, Ju-Hee; Choi, Susanna; Lee, Jae-Eun; Hur, On-Sook; Ro, Na-Young; Hwang, Ae-Jin; Ko, Ho-Cheol; Chung, Yun-Jo; Noh, Jae-Jong; Assefa, Awraris Derbie.
Afiliação
  • Rhee JH; National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Korea.
  • Choi S; National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Korea.
  • Lee JE; National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Korea.
  • Hur OS; National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Korea.
  • Ro NY; National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Korea.
  • Hwang AJ; National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Korea.
  • Ko HC; Client Service Division, Planning and Coordination Bureau, RDA, Jeonju 54875, Korea.
  • Chung YJ; National Creative Research Laboratory for Ca2+ signaling Network, Jeonbuk National University Medical School, Jeonju, 54896, Korea.
  • Noh JJ; Jeonbuk Agricultural Research and Extension Services, Iksan 54591, Korea.
  • Assefa AD; National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Korea.
Plants (Basel) ; 9(11)2020 Oct 23.
Article em En | MEDLINE | ID: mdl-33114129
ABSTRACT
Glucosinolates (GSLs) are sulfur-containing secondary metabolites naturally occurring in Brassica species. The purpose of this study was to identify the GSLs, determine their content, and study their accumulation patterns within and between leaves of kimchi cabbage (Brassica rapa L.) cultivars. GSLs were analyzed using UPLC-MS/MS in negative electron-spray ionization (ESI-) and multiple reaction monitoring (MRM) mode. The total GSL content determined in this study ranged from 621.15 to 42434.21 µmolkg-1 DW. Aliphatic GSLs predominated, representing from 4.44% to 96.20% of the total GSL content among the entire samples. Glucobrassicanapin (GBN) contributed the greatest proportion while other GSLs such as glucoerucin (ERU) and glucotropaeolin (TRO) were found in relatively low concentrations. Principal component analysis (PCA) yielded three principal components (PCs) with eigenvalues ≥ 1, altogether representing 74.83% of the total variation across the entire dataset. Three kimchi cabbage (S/No. 20, 4, and 2), one leaf mustard (S/No. 26), and one turnip (S/No. 8) genetic resources were well distinguished from other samples. The GSL content varied significantly among the different positions (outer, middle, and inner) of the leaves and sections (top, middle, bottom, green/red, and white) within the leaves. In most of the samples, higher GSL content was observed in the proximal half and white sections and the middle layers of the leaves. GSLs are regarded as allelochemicals; hence, the data related to the patterns of GSLs within the leaf and between leaves at a different position could be useful to understand the defense mechanism of Brassica plants. The observed variability could be useful for breeders to develop Brassica cultivars with high GSL content or specific profiles of GSLs.
Palavras-chave

Texto completo: 1 Bases de dados: MEDLINE Idioma: En Revista: Plants (Basel) Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Bases de dados: MEDLINE Idioma: En Revista: Plants (Basel) Ano de publicação: 2020 Tipo de documento: Article