Repurposing a microfluidic formulation device for automated DNA construction.
PLoS One
; 15(11): e0242157, 2020.
Article
em En
| MEDLINE
| ID: mdl-33175889
ABSTRACT
Microfluidic applications have expanded greatly over the past decade. For the most part, however, each microfluidics platform is developed with a specific task in mind, rather than as a general-purpose device with a wide-range of functionality. Here, we show how a microfluidic system, originally developed to investigate protein phase behavior, can be modified and repurposed for another application, namely DNA construction. We added new programable controllers to direct the flow of reagents across the chip. We designed the assembly of a combinatorial Golden Gate DNA library using TeselaGen DESIGN software and used the repurposed microfluidics platform to assemble the designed library from off-chip prepared DNA assembly pieces. Further experiments verified the sequences and function of the on-chip assembled DNA constructs.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
DNA
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Microfluídica
/
Dispositivos Lab-On-A-Chip
Idioma:
En
Revista:
PLoS One
Assunto da revista:
CIENCIA
/
MEDICINA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos