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Generation of immune cell containing adipose organoids for in vitro analysis of immune metabolism.
Taylor, Jacqueline; Sellin, Julia; Kuerschner, Lars; Krähl, Lennart; Majlesain, Yasmin; Förster, Irmgard; Thiele, Christoph; Weighardt, Heike; Weber, Elvira.
Afiliação
  • Taylor J; Biochemistry and Cell Biology of Lipids, Life and Medical Sciences Institute (LIMES), University of Bonn, Carl-Troll-Straße 31, 53115, Bonn, Germany.
  • Sellin J; Division Vascular Signaling and Cancer (A270), German Cancer Research Center (DKFZ), 69120, Heidelberg, Germany.
  • Kuerschner L; Faculty of Biosciences, University of Heidelberg, 69120, Heidelberg, Germany.
  • Krähl L; ZSEB, Center for Rare Diseases, University Hospital Bonn, 53127, Bonn, Germany.
  • Majlesain Y; Biochemistry and Cell Biology of Lipids, Life and Medical Sciences Institute (LIMES), University of Bonn, Carl-Troll-Straße 31, 53115, Bonn, Germany.
  • Förster I; Immunology and Environment, Life and Medical Sciences (LIMES) Institute, University of Bonn, Carl-Troll-Straße 31, 53115, Bonn, Germany.
  • Thiele C; Immunology and Environment, Life and Medical Sciences (LIMES) Institute, University of Bonn, Carl-Troll-Straße 31, 53115, Bonn, Germany.
  • Weighardt H; Immunology and Environment, Life and Medical Sciences (LIMES) Institute, University of Bonn, Carl-Troll-Straße 31, 53115, Bonn, Germany.
  • Weber E; Biochemistry and Cell Biology of Lipids, Life and Medical Sciences Institute (LIMES), University of Bonn, Carl-Troll-Straße 31, 53115, Bonn, Germany.
Sci Rep ; 10(1): 21104, 2020 12 03.
Article em En | MEDLINE | ID: mdl-33273595
Adipose tissue is an organized endocrine organ with important metabolic and immunological functions and immune cell-adipocyte crosstalk is known to drive various disease pathologies. Suitable 3D adipose tissue organoid models often lack resident immune cell populations and therefore require the addition of immune cells isolated from other organs. We have created the first 3D adipose tissue organoid model which could contain and maintain resident immune cell populations of the stromal vascular fraction (SVF) and proved to be effective in studying adipose tissue biology in a convenient manner. Macrophage and mast cell populations were successfully confirmed within our organoid model and were maintained in culture without the addition of growth factors. We demonstrated the suitability of our model for monitoring the lipidome during adipocyte differentiation in vitro and confirmed that this model reflects the physiological lipidome better than standard 2D cultures. In addition, we applied mass spectrometry-based lipidomics to track lipidomic changes in the lipidome upon dietary and immunomodulatory interventions. We conclude that this model represents a valuable tool for immune-metabolic research.
Assuntos

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Organoides / Tecido Adiposo Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Organoides / Tecido Adiposo Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Alemanha