Toward whole tissue imaging of axolotl regeneration.
Dev Dyn
; 250(6): 800-806, 2021 06.
Article
em En
| MEDLINE
| ID: mdl-33336514
ABSTRACT
The axolotl is a highly regenerative organism and has been studied in laboratories for over 150 years. Despite a long-standing fascination with regeneration in general and axolotl specifically, we are still scratching the surface trying to visualize and understand the complex cellular behavior that underlies axolotl regeneration. In this review, we will discuss the progress that has been made in visualizing these processes focusing on four major aspects cell labeling approaches, the removal of pigmentation, reductionist approaches to perform live cell imaging, and finally recent developments applying tissue clearing strategies to visualize the processes that underly regeneration. We also provide several suggestions that the community could consider exploring, notably the generation of novel alleles that further reduce pigmentation as well as improvements in tissue clearing strategies.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Regeneração
/
Diagnóstico por Imagem
/
Ambystoma mexicanum
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Dev Dyn
Assunto da revista:
ANATOMIA
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Áustria