Masseter response to long-term experimentally induced anterior crossbite in Sprague-Dawley rats.
Arch Oral Biol
; 122: 104985, 2021 Feb.
Article
em En
| MEDLINE
| ID: mdl-33340921
ABSTRACT
OBJECTIVE:
To detect the long-term response to unilateral anterior crossbite (UAC) in masticatory muscles and in molecular biomarkers of peripheral blood leukocytes.DESIGN:
Fifty-six six-week-old Sprague-Dawley rats were used. The gene-fold changes in peripheral blood leukocytes were detected by the microarray analysis to compare the rats that received 20-week UAC treatment with age-matched controls (n = 4). Muscle atrophy-related gene Fbxo32 was selected based on the data of the microarray analysis verified by using real-time PCR. The remaining 36 rats were randomly separated in the UAC and control groups at 12 and 20 weeks (n = 12). The protein expression of Fbxo32 and the muscle injury and myogenesis-related markers, αB-crystallin and desmin, were detected in the masseter and lateral pterygoid muscles by western blot assay.RESULTS:
In the 20-week UAC group, the masseter muscle weight was lower than that in the age-matched control group, and the expression level of Fbxo32 gene in peripheral blood leukocytes was increased according to the microarray analysis confirmed by real-time PCR detection. The increased protein expression levels of Fbxo32 were detected in the masseter in the 20-week UAC group, and the protein expression levels of desmin and αB-crystallin were decreased at this time point. No similar changes were detected in the lateral pterygoid muscle.CONCLUSIONS:
Masseter atrophy is induced by long-term stimulation of UAC. The increased expression of the Fbxo32 gene in peripheral blood leukocytes may be a candidate biological marker of masseter atrophy.Palavras-chave
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Bases de dados:
MEDLINE
Assunto principal:
Má Oclusão
/
Músculo Masseter
Limite:
Animals
Idioma:
En
Revista:
Arch Oral Biol
Ano de publicação:
2021
Tipo de documento:
Article