The role of disorder in RNA binding affinity and specificity.
Open Biol
; 10(12): 200328, 2020 12.
Article
em En
| MEDLINE
| ID: mdl-33352065
ABSTRACT
Most RNA-binding modules are small and bind few nucleotides. RNA-binding proteins typically attain the physiological specificity and affinity for their RNA targets by combining several RNA-binding modules. Here, we review how disordered linkers connecting RNA-binding modules govern the specificity and affinity of RNA-protein interactions by regulating the effective concentration of these modules and their relative orientation. RNA-binding proteins also often contain extended intrinsically disordered regions that mediate protein-protein and RNA-protein interactions with multiple partners. We discuss how these regions can connect proteins and RNA resulting in heterogeneous higher-order assemblies such as membrane-less compartments and amyloid-like structures that have the characteristics of multi-modular entities. The assembled state generates additional RNA-binding specificity and affinity properties that contribute to further the function of RNA-binding proteins within the cellular environment.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
RNA
/
Proteínas de Ligação a RNA
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Open Biol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos