Effective Treatment of Cutaneous Mold Infections by Antimicrobial Blue Light That Is Potentiated by Quinine.
J Infect Dis
; 224(6): 1069-1076, 2021 09 17.
Article
em En
| MEDLINE
| ID: mdl-33528496
ABSTRACT
BACKGROUND:
Cutaneous mold infections commonly result from an array of traumatic injuries that involve direct inoculation of contaminated soil into wounds. Here, we explored the use of antimicrobial blue light (aBL; 405 nm wavelength) and the combination of aBL with quinine hydrochloride (aBLâ +â Q-HCL) for the treatment of cutaneous mold infections.METHODS:
Efficacy of aBL and aBLâ +â Q-HCL in killing clinically important pathogenic molds (Aspergillus fumigatus, Aspergillus flavus, and Fusarium oxyprorum) was investigated. Ultraperformance liquid chromatography identified and quantified endogenous porphyrins in the mold conidia. Finally, a mouse model of dermabrasion wound infected with a bioluminescent variant of A. fumigatus was developed to investigate the efficacy of aBL in treating cutaneous mold infections.RESULTS:
We demonstrated that mold conidia are tolerant to aBL, but Q-HCL enhances efficacy. Transmission electron microscopy revealed intracellular damage by aBL. aBLâ +â Q-HCL resulted in intracellular and cell wall damage. Porphyrins were observed in all mold strains, with A. fumigatus having the highest concentration. aBL and aBLâ +â Q-HCL effectively reduced the burden of A. fumigatus within an established dermabrasion infection and limited recurrence posttreatment.CONCLUSIONS:
aBL and aBLâ +â Q-HCL may offer a novel approach for the treatment of mold infections.Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Porfirinas
/
Quinina
/
Aspergillus fumigatus
/
Dermatopatias Infecciosas
/
Antibacterianos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Infect Dis
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos