Variation in the relative synthesis of some proteins in mammalian cells exposed to hypertonic medium.

ABSTRACT

Exposure of a number of quiescent murine and human cell lines to low-graded doses of cycloheximide (CXM) results in a pattern of protein synthesis consisting of enhanced and induced species. This pattern is reminiscent of but not identical to that observed after several stress treatments [V. Sorrentino et al. (1985) J. Cell. Physiol. 125, 313]. A pattern identical to that seen after exposure to CXM is synthesized when cells are exposed to an hypertonic growth medium resulting in a full and reversible block of the initiation of polypeptide chain. This suggests that this kind of response is triggered by a reduction of overall protein synthesis rather than by a slow-down of the elongation step. Analysis of the synthesis of histones and ribosomal proteins during these two nonphysiological treatments (CXM or high salt) shows that these classes of proteins are neither stimulated nor preferentially retained. In contrast, greatly enhanced levels of steady-state histone mRNAs have been observed which have been translated in a reticulocyte lysate system, but are not apparently translated in vivo.