CRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering.
Sci Rep
; 11(1): 6796, 2021 03 24.
Article
em En
| MEDLINE
| ID: mdl-33762639
ABSTRACT
Genome engineering of bacteriophages provides opportunities for precise genetic dissection and for numerous phage applications including therapy. However, few methods are available for facile construction of unmarked precise deletions, insertions, gene replacements and point mutations in bacteriophages for most bacterial hosts. Here we describe CRISPY-BRED and CRISPY-BRIP, methods for efficient and precise engineering of phages in Mycobacterium species, with applicability to phages of a variety of other hosts. This recombineering approach uses phage-derived recombination proteins and Streptococcus thermophilus CRISPR-Cas9.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Bacteriófagos
/
Engenharia Genética
/
Sistemas CRISPR-Cas
Idioma:
En
Revista:
Sci Rep
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos