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Evaluation of the Roche MagNA Pure 96 nucleic acid extraction platform for the Seegene Anyplex II HPV28 detection assay.
Atchison, S; Shilling, H; Balgovind, P; Machalek, D A; Hawkes, D; Garland, S M; Saville, M; Murray, G; Molano, M; Danielewski, J; Phillips, S.
Afiliação
  • Atchison S; Centre for Women's Infectious Diseases, The Royal Women's Hospital, Melbourne, Vic, Australia.
  • Shilling H; Murdoch Children's Research Institute, Melbourne, Vic, Australia.
  • Balgovind P; Centre for Women's Infectious Diseases, The Royal Women's Hospital, Melbourne, Vic, Australia.
  • Machalek DA; Murdoch Children's Research Institute, Melbourne, Vic, Australia.
  • Hawkes D; Centre for Women's Infectious Diseases, The Royal Women's Hospital, Melbourne, Vic, Australia.
  • Garland SM; Murdoch Children's Research Institute, Melbourne, Vic, Australia.
  • Saville M; Centre for Women's Infectious Diseases, The Royal Women's Hospital, Melbourne, Vic, Australia.
  • Murray G; Melbourne School of Population and Global Health, University of Melbourne, Melbourne, Vic, Australia.
  • Molano M; The Kirby Institute, UNSW Sydney, Sydney, NSW, Australia.
  • Danielewski J; VCS Foundation, Melbourne, Vic, Australia.
  • Phillips S; Department of Biochemistry and Pharmacology, University of Melbourne, Melbourne, Vic, Australia.
J Appl Microbiol ; 131(5): 2592-2599, 2021 Nov.
Article em En | MEDLINE | ID: mdl-33942451
AIM: Validate the Roche, MagNAPure96 (MP96) nucleic acid extraction platform for Seegene Anyplex II HPV28 (Anyplex28) detection of Human Papillomavirus. METHODS AND RESULTS: Comparisons were made for Anyplex28 genotyping from 115 cervical samples extracted on the Hamilton, STARlet and the MP96. Two DNA concentrations were used for the MP96, one matched for sample input to the STARlet and another 5× concentration (laboratory standard). Agreement of HPV detection was 89·8% (κ = 0·798; P = 0·007), with HPV detected in 10 more samples for the MP96. There was a high concordance of detection for any oncogenic HPV genotype (κ = 0·77; P = 0·007) and for any low-risk HPV genotype (κ = 0·85; P = 0·008). DNA extracted at laboratory standard had a lower overall agreement 85·2% (κ = 0·708; P < 0·001), with 17/115 discordant positive samples that tested negative after STARlet extraction. Of the discordant genotypes, 72·7% were detected in the lowest signal range for Anyplex28 ('+'). CONCLUSIONS: MP96 performed with high concordance to STARlet, although produced DNA with a higher analytical sensitivity on the Anyplex28. SIGNIFICANCE AND IMPACT OF THE STUDY: This analysis supports the use of samples extracted on the MP96 for HPV genotyping using the Anyplex28. Furthermore, an increase in DNA concentration increased analytical sensitivity of the Anyplex28, particularly appropriate for prevalence studies.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Ácidos Nucleicos / Infecções por Papillomavirus Tipo de estudo: Diagnostic_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: J Appl Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Austrália

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Ácidos Nucleicos / Infecções por Papillomavirus Tipo de estudo: Diagnostic_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: J Appl Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Austrália