Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins.
Methods Mol Biol
; 2305: 193-201, 2021.
Article
em En
| MEDLINE
| ID: mdl-33950391
ABSTRACT
In this chapter, we describe the preparatory and spectroscopic procedures for conducting solid-state NMR experiments on microtubules (MTs) obtained from human cells and their complexes with microtubule-associated proteins (MAPs). Next to labeling and functional assembly of MTs and MT-MAP complexes, we discuss solid-state NMR approaches, including fast MAS and hyperpolarization methods that can be used to examine these systems. Such studies can provide novel insight into the dynamic properties of MTs and MT-MAP complexes.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Tubulina (Proteína)
/
Espectroscopia de Ressonância Magnética
/
Proteínas Associadas aos Microtúbulos
/
Microtúbulos
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Holanda