Your browser doesn't support javascript.
loading
Changes in the mesocorticolimbic pathway after low dose reserpine-treatment in Wistar and Spontaneously Hypertensive Rats (SHR): Implications for cognitive deficits in a progressive animal model for Parkinson's disease.
Leão, Anderson H F F; Meurer, Ywlliane S R; Freitas, Thalma A; Medeiros, André M; Abílio, Vanessa C; Izídio, Geison S; Conceição, Isaltino M; Ribeiro, Alessandra M; Silva, Regina H.
Afiliação
  • Leão AHFF; Department of Pharmacology, Universidade Federal de São Paulo, São Paulo, Brazil.
  • Meurer YSR; Memory and Cognition Laboratory, Department of Psychology, Universidade Federal da Paraíba, João Pessoa, Brazil.
  • Freitas TA; Department of Pharmacology, Universidade Federal de São Paulo, São Paulo, Brazil; Laboratory of Pharmacology, Group of Toxin Action Mode (MATx), Butantan Institute, São Paulo, Brazil.
  • Medeiros AM; Center of Health and Biological Sciences, Universidade Federal Rural do Semi-árido, Mossoró, Brazil.
  • Abílio VC; Department of Pharmacology, Universidade Federal de São Paulo, São Paulo, Brazil; National Institute for Translational Medicine (INCT-TM, CNPq), Ribeirão Preto, Brazil.
  • Izídio GS; Laboratory of Behavioral Genetics, Department of Cellular Biology, Embryology and Genetics, Universidade Federal de Santa Catarina, Florianopolis, Brazil.
  • Conceição IM; Laboratory of Pharmacology, Group of Toxin Action Mode (MATx), Butantan Institute, São Paulo, Brazil.
  • Ribeiro AM; Department of Biosciences, Universidade Federal de São Paulo, Santos, Brazil.
  • Silva RH; Department of Pharmacology, Universidade Federal de São Paulo, São Paulo, Brazil. Electronic address: reginahsilva@gmail.com.
Behav Brain Res ; 410: 113349, 2021 07 23.
Article em En | MEDLINE | ID: mdl-33971246
ABSTRACT
Reserpine (RES) is an irreversible inhibitor of VMAT2 used to study Parkinson's disease (PD) and screening for antiparkinsonian treatments in rodents. Recently, the repeated treatment with a low dose of reserpine was proposed as a model capable of emulating progressive neurochemical, motor and non-motor impairments in PD. Conversely, compared to Wistar rats, Spontaneously Hypertensive Rats (SHR) are resistant to motor changes induced by repeated treatment with a low dose of RES. However, such resistance has not yet been investigated for RES-induced non-motor impairments. We aimed to assess whether SHR would have differential susceptibility to the object recognition deficit induced by repeated low-dose reserpine treatment. We submitted male Wistar and SHR rats to repeated RES treatment (15 s.c. injections of 0.1 mg/kg, every other day) and assessed object memory acquisition and retrieval 48 h after the 6th RES injection (immediately before the appearance of motor impairments). Only RES Wistar rats displayed memory impairment after reserpine treatment. On the other hand, untreated SHR rats displayed object recognition memory deficit, but RES treatment restored such deficits. We also performed immunohistochemistry for tyrosine hydroxylase (TH) and α-synuclein (α-syn) 48 h after the last RES injection. In a different set of animals submitted to the same treatment, we quantified DA, 5-HT and products of lipid peroxidation in the prefrontal cortex (PFC) and hippocampus (HPC). SHR presented increased constitutive levels of DA in the PFC and reduced immunoreactivity to TH in the medial PFC and dorsal HPC. Corroborating the behavioral findings, RES treatment restored those constitutive alterations in SHR. These findings indicate that the neurochemical, molecular and genetic differences in the SHR strain are potentially relevant targets to the study of susceptibility to diseases related to dopaminergic alterations.
Assuntos
Palavras-chave

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Doença de Parkinson Secundária / Reserpina / Tirosina 3-Mono-Oxigenase / Dopamina / Córtex Pré-Frontal / Transtornos Cognitivos / Reconhecimento Psicológico / Proteínas Vesiculares de Transporte de Monoamina / Hipocampo Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Behav Brain Res Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Doença de Parkinson Secundária / Reserpina / Tirosina 3-Mono-Oxigenase / Dopamina / Córtex Pré-Frontal / Transtornos Cognitivos / Reconhecimento Psicológico / Proteínas Vesiculares de Transporte de Monoamina / Hipocampo Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Behav Brain Res Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil