Efficient generation of isogenic primary human myeloid cells using CRISPR-Cas9 ribonucleoproteins.
Cell Rep
; 35(6): 109105, 2021 05 11.
Article
em En
| MEDLINE
| ID: mdl-33979618
ABSTRACT
Genome engineering of primary human cells with CRISPR-Cas9 has revolutionized experimental and therapeutic approaches to cell biology, but human myeloid-lineage cells have remained largely genetically intractable. We present a method for the delivery of CRISPR-Cas9 ribonucleoprotein (RNP) complexes by nucleofection directly into CD14+ human monocytes purified from peripheral blood, leading to high rates of precise gene knockout. These cells can be efficiently differentiated into monocyte-derived macrophages or dendritic cells. This process yields genetically edited cells that retain transcript and protein markers of myeloid differentiation and phagocytic function. Genetic ablation of the restriction factor SAMHD1 increased HIV-1 infection >50-fold, demonstrating the power of this system for genotype-phenotype interrogation. This fast, flexible, and scalable platform can be used for genetic studies of human myeloid cells in immune signaling, inflammation, cancer immunology, host-pathogen interactions, and beyond, and could facilitate the development of myeloid cellular therapies.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Ribonucleoproteínas
/
Genoma
/
Células Mieloides
/
Sistemas CRISPR-Cas
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Cell Rep
Ano de publicação:
2021
Tipo de documento:
Article