RNF8 is not required for histone-to-protamine exchange in spermiogenesis.
Biol Reprod
; 105(5): 1154-1159, 2021 11 15.
Article
em En
| MEDLINE
| ID: mdl-34225362
ABSTRACT
While an E3 ubiquitin ligase, RNF8, was initially reported to be required for histone-to-protamine exchange in spermiogenesis, we subsequently demonstrated that RNF8 is not involved in this process. Nevertheless, reflecting a lingering misunderstanding in the field, a growing number of studies have continued to postulate a requirement for RNF8 in the histone-to-protamine exchange. For example, a recent study claimed that a mouse PIWI protein, MIWI, controls RNF8-mediated histone-to-protamine exchange. Here, confirming our earlier conclusions, we show that RNF8 is required neither for the establishment of histone H4K16 acetylation, which is an initial step in histone removal during spermiogenesis, nor for the incorporation of two protamine proteins, PRM1 and PRM2. Thus, whereas RNF8 mediates ubiquitination of H2A on the sex chromosomes in meiosis, during the prior stage of spermatogenesis, our genetic evidence underscores that RNF8 is not involved in histone-to-protamine exchange.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Espermatogênese
/
Histonas
/
Protaminas
/
Ubiquitina-Proteína Ligases
Limite:
Animals
Idioma:
En
Revista:
Biol Reprod
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos