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Simple, fast and efficient iTOP-mediated delivery of CRISPR/Cas9 RNP in difficult-to-transduce human cells including primary T cells.
Kholosy, Waleed M; Visscher, Marieke; Ogink, Kim; Buttstedt, Helen; Griffin, Kelli; Beier, Axel; Gerlach, Jan Patrick; Molenaar, Jan J; Geijsen, Niels; de Boer, Marco; Chatsisvili, Anna.
Afiliação
  • Kholosy WM; Princess Máxima Center for Pediatric Oncology, Heidelberglaan 25, 3584 CS Utrecht, the Netherlands.
  • Visscher M; NTrans Technologies BV, Heidelberglaan 7, 3584 CS Utrecht, the Netherlands.
  • Ogink K; NTrans Technologies BV, Heidelberglaan 7, 3584 CS Utrecht, the Netherlands.
  • Buttstedt H; NTrans Technologies BV, Heidelberglaan 7, 3584 CS Utrecht, the Netherlands.
  • Griffin K; NTrans Technologies BV, Heidelberglaan 7, 3584 CS Utrecht, the Netherlands.
  • Beier A; KNAW-Hubrecht Institute, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands.
  • Gerlach JP; NTrans Technologies BV, Heidelberglaan 7, 3584 CS Utrecht, the Netherlands.
  • Molenaar JJ; Princess Máxima Center for Pediatric Oncology, Heidelberglaan 25, 3584 CS Utrecht, the Netherlands.
  • Geijsen N; KNAW-Hubrecht Institute, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands.
  • de Boer M; NTrans Technologies BV, Heidelberglaan 7, 3584 CS Utrecht, the Netherlands.
  • Chatsisvili A; NTrans Technologies BV, Heidelberglaan 7, 3584 CS Utrecht, the Netherlands. Electronic address: a.chatsisvili@uu.nl.
J Biotechnol ; 338: 71-80, 2021 Sep 10.
Article em En | MEDLINE | ID: mdl-34271056

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Linfócitos T / Sistemas CRISPR-Cas Tipo de estudo: Guideline Limite: Humans Idioma: En Revista: J Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Holanda

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Linfócitos T / Sistemas CRISPR-Cas Tipo de estudo: Guideline Limite: Humans Idioma: En Revista: J Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Holanda