Giant Y79 retinoblastoma cells contain functionally active T-type calcium channels.
Pflugers Arch
; 473(10): 1631-1639, 2021 10.
Article
em En
| MEDLINE
| ID: mdl-34392423
ABSTRACT
Retinoblastoma is the most common malignant intraocular tumor in children. Y79 human retinoblastoma cells are in vitro models of retinal tumors used for drug screening. Undifferentiated Y79 cells originate from a primitive multi-potential neuroectodermal cell and express neuronal and glial properties. However, the nature of cellular heterogeneity in Y79 cells is unclear because functional methods to characterize neurons or glial cells have not been employed to Y79 cells. Here, we perform patch-clamp recordings to characterize electrophysiological properties in retinoblastoma cells. We identified a population of large-sized Y79 cells (i.e., giant cells, ~ 40-µm diameter), hyperpolarized resting membrane potential (-54 mV), and low input resistance (~ 600 MΩ), indicating electrically mature cells. We also found that giant Y79 cells contain increased density of T-type calcium channels. Finally, we found that T-type calcium channels are active only in giant cells suggesting that cancer treatments aimed to prevent calcium influx in retinoblastomas should be tested in giant cells.
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MEDLINE
Assunto principal:
Retinoblastoma
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Células Gigantes
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Neoplasias da Retina
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Canais de Cálcio Tipo T
Limite:
Humans
Idioma:
En
Revista:
Pflugers Arch
Ano de publicação:
2021
Tipo de documento:
Article