Cinnamomum zeylanicum Blume (Ceylon cinnamon) bark extract attenuates doxorubicin induced cardiotoxicity in Wistar rats.
Saudi Pharm J
; 29(8): 820-832, 2021 Aug.
Article
em En
| MEDLINE
| ID: mdl-34408544
ABSTRACT
Anti-tumour efficacy of doxorubicin is hindered by the cumulative dose-dependent cardiotoxicity induced by reactive oxygen species during its metabolism. As Cinnamomum zeylanicum has proven antioxidant potential, objective of this study was to investigate the cardioprotective activity of Cinnamomum bark extract against doxorubicin induced cardiotoxicity in Wistar rats. Physicochemical and phytochemical analysis was carried out and dose response effect and the cardioprotective activity of Cinnamomum were determined in vivo. 180 mg/kg dexrazoxane was used as the positive control. Plant extracts were free of heavy metals and toxic phytoconstituents. In vivo study carried out in Wistar rats revealed a significant increase (p < 0.05) in cardiac troponin I, NT-pro brain natriuretic peptide, AST and LDH concentrations in the doxorubicin control group (18 mg/kg) compared to the normal control. Rats pre-treated with the optimum dosage of Cinnmamomum (2.0 g/kg) showed a significant reduction (p < 0.05) in all above parameters compared to the doxorubicin control. A significant reduction was observed in the total antioxidant capacity, reduced glutathione, glutathione peroxidase, glutathione reductase, superoxide dismutase and catalase activity while the lipid peroxidation and myeloperoxidase activity were significantly increased in the doxorubicin control group compared to the normal control (p < 0.05). Pre-treatment with Cinnamomum bark showed a significant decrease in lipid peroxidation, myeloperoxidase activity and significant increase in rest of the parameters compared to the doxorubicin control (p < 0.05). Histopathological analysis revealed a preserved appearance of the myocardium and lesser degree of cellular changes of necrosis in rats pre-treated with Cinnamomum extract. In conclusion, Cinnamomum bark extract has the potential to significantly reduce doxorubicin induced oxidative stress and inflammation in Wistar rats.
ABEC, Aqueous bark extract of Cinnamomum zeylanicum; ABTS, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid); AST, Aspartate aminotransferase; Antioxidant effect; Cardiotoxicity; Cinnamomum zeylanicum bark extract; DNA, Deoxyribonucleic acid; DPPH, 2,2-diphenyl-1-picrylhydrazyl; Doxorubicin; ELISA, Enzyme-linked immunosorbent assay; FRAP, Ferric reducing antioxidant power; GPx, Glutathione peroxidase; GR, Glutathione reductase; GSH, Reduced glutathione; H & E, Haematoxylin and eosin; IP, Intraperitoneal; LDH, Lactate dehydrogenase; MDA, Malondialdehyde; MPO, Myeloperoxidase; Myeloperoxidase; NADPH, Nicotinamide adenine dinucleotide phosphate hydrogen; NO, Nitric oxide; NT-pro BNP, N terminal- pro brain natriuretic peptide; Oxidative-stress; PBS, Phosphate buffered saline; ROS, Reactive oxygen species; SOD, Superoxide dismutase; USA, United States of America; WHO, World Health Organization; cTnI, Cardiac troponin I
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Bases de dados:
MEDLINE
Idioma:
En
Revista:
Saudi Pharm J
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Sri Lanka