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Tanshinone IIA protects human coronary artery endothelial cells from ferroptosis by activating the NRF2 pathway.
He, Lina; Liu, Ying-Yi; Wang, Kun; Li, Chengxi; Zhang, Weibin; Li, Zhen-Zhen; Huang, Xian-Zhang; Xiong, Yujuan.
Afiliação
  • He L; The Second Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, 510220, China. Electronic address: 20191110229@stu.gzucm.edu.cn.
  • Liu YY; The Cardiovascular Hospital of Shanxi, Taiyuan, 030000, China. Electronic address: lyy4993@hotmail.com.
  • Wang K; Research Center of Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China. Electronic address: wangkun@gzucm.edu.cn.
  • Li C; Research Center of Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China. Electronic address: 2017101027@stu.gzucm.edu.cn.
  • Zhang W; Research Center of Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China. Electronic address: 2017101016@stu.gzucm.edu.cn.
  • Li ZZ; Department of Laboratory Medicine, Panyu Hospital of Chines Medicine, Guangzhou University of Chinese Medicine, 511400, China. Electronic address: lizhenzhen2727@hotmail.com.
  • Huang XZ; Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Key Laboratory of Research on Emergency in TCM, Guangzhou, 510120, China. Electronic address: huangxz020@gzucm.edu.cn.
  • Xiong Y; Department of Laboratory Medicine, Panyu Hospital of Chines Medicine, Guangzhou University of Chinese Medicine, 511400, China. Electronic address: yujuanxiong@gzucm.edu.cn.
Biochem Biophys Res Commun ; 575: 1-7, 2021 10 20.
Article em En | MEDLINE | ID: mdl-34454174
ABSTRACT
The pathogenesis of atherosclerosis is closely related to endothelial cell injury caused by lipid peroxidation-induced ferroptosis. Tanshinone IIA (TSA) protects endothelial tissues from damage. In this study, we investigated whether TSA exerts its protective effect on endothelial cells by inhibiting ferroptosis. Ferroptosis was induced in human coronary artery endothelial cells (HCAECs), and cells were treated with TSA. Morphological examination indicated that TSA exerted a significant protective effect on the HCAECs. This was further confirmed by LDH release and cell death detection assays. Flow cytometry revealed that TSA significantly reduced the excessive accumulation of total cellular ROS and lipid ROS caused by ferroptosis inducers. TSA also restored the reduction of glutathione (GSH), a potent and abundant reductant in cells. In addition, we found that TSA promoted the expression of NRF2, an essential player in response to oxidative stress, and its downstream genes. Immunofluorescent staining revealed that TSA promoted the nuclear translocation of NRF2. Increased nuclear translocation of NRF2 was validated by Western blot evaluation of cytoplasmic and nuclear protein extracts. Furthermore, NRF2 inhibition abolished the protective effects of TSA on HCAECs. These data demonstrate that TSA represses ferroptosis via activation of NRF2 in HCAECs.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Peroxidação de Lipídeos / Vasos Coronários / Células Endoteliais / Abietanos / Aterosclerose / Fator 2 Relacionado a NF-E2 / Ferroptose Limite: Humans Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Peroxidação de Lipídeos / Vasos Coronários / Células Endoteliais / Abietanos / Aterosclerose / Fator 2 Relacionado a NF-E2 / Ferroptose Limite: Humans Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2021 Tipo de documento: Article