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Distinct neuropeptide-receptor modules regulate a sex-specific behavioral response to a pheromone.
Reilly, Douglas K; McGlame, Emily J; Vandewyer, Elke; Robidoux, Annalise N; Muirhead, Caroline S; Northcott, Haylea T; Joyce, William; Alkema, Mark J; Gegear, Robert J; Beets, Isabel; Srinivasan, Jagan.
Afiliação
  • Reilly DK; Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA, USA.
  • McGlame EJ; Tufts University, Medford, MA, USA.
  • Vandewyer E; Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA, USA.
  • Robidoux AN; AbbVie Foundational Neuroscience Center, Cambridge, MA, USA.
  • Muirhead CS; Neural Signaling and Circuit Plasticity Group, Department of Biology, KU Leuven, Leuven, Belgium.
  • Northcott HT; Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA, USA.
  • Joyce W; Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA, USA.
  • Alkema MJ; Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA, USA.
  • Gegear RJ; Optum, Hartford, CT, USA.
  • Beets I; Neurobiology Department, University of Massachusetts Medical School, Worcester, MA, USA.
  • Srinivasan J; Neurobiology Department, University of Massachusetts Medical School, Worcester, MA, USA.
Commun Biol ; 4(1): 1018, 2021 08 31.
Article em En | MEDLINE | ID: mdl-34465863
Dioecious species are a hallmark of the animal kingdom, with opposing sexes responding differently to identical sensory cues. Here, we study the response of C. elegans to the small-molecule pheromone, ascr#8, which elicits opposing behavioral valences in each sex. We identify a novel neuropeptide-neuropeptide receptor (NP/NPR) module that is active in males, but not in hermaphrodites. Using a novel paradigm of neuropeptide rescue that we established, we leverage bacterial expression of individual peptides to rescue the sex-specific response to ascr#8. Concurrent biochemical studies confirmed individual FLP-3 peptides differentially activate two divergent receptors, NPR-10 and FRPR-16. Interestingly, the two of the peptides that rescued behavior in our feeding paradigm are related through a conserved threonine, suggesting that a specific NP/NPR combination sets a male state, driving the correct behavioral valence of the ascr#8 response. Receptor expression within pre-motor neurons reveals novel coordination of male-specific and core locomotory circuitries.
Assuntos

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Caenorhabditis elegans / Receptores de Neuropeptídeos / Organismos Hermafroditas / Locomoção Limite: Animals Idioma: En Revista: Commun Biol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Caenorhabditis elegans / Receptores de Neuropeptídeos / Organismos Hermafroditas / Locomoção Limite: Animals Idioma: En Revista: Commun Biol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos