Thermostability Measurement of an α-Glucosidase Using a Classical Activity-based Assay and a Novel Thermofluor Method.
Bio Protoc
; 7(12): e2349, 2017 Jun 20.
Article
em En
| MEDLINE
| ID: mdl-34541099
ABSTRACT
α-glucosidases (including maltases and isomaltases) are enzymes which release glucose from a set of α-glucosidic substrates. Their catalytic activity, substrate specificity and thermostability can be assayed using this trait. Thermostability of proteins can also be determined using a high-throughput differential scanning fluorometry method, also named Thermofluor. We have shown that Thermofluor can also be applied to predict binding of substrates and inhibitors to a yeast α-glucosidase. The methods described here in detail were used in Viigand et al., 2016 .
Texto completo:
1
Bases de dados:
MEDLINE
Idioma:
En
Revista:
Bio Protoc
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estônia