An optimized ATAC-seq protocol for genome-wide mapping of active regulatory elements in primary mouse cortical neurons.
STAR Protoc
; 2(4): 100854, 2021 12 17.
Article
em En
| MEDLINE
| ID: mdl-34647036
ABSTRACT
ATAC-seq is a versatile, adaptable, and widely adopted technique for mapping open chromatin regions. However, some biological systems, such as primary neurons, present unique challenges to its application. Conventional ATAC-seq would require the dissociation of the primary neurons after plating but dissociating them leads to rapid cell death and major changes in cell state, affecting ATAC-seq results. We have developed this modified ATAC-seq protocol to address this challenge for primary neurons, providing a high-quality and high-resolution accessible chromatin profile. For complete details on the use and execution of this protocol, please refer to Maor-Nof et al. (2021).
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Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Sequenciamento de Nucleotídeos em Larga Escala
/
Sequenciamento de Cromatina por Imunoprecipitação
Limite:
Animals
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos