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A standarized protocol for evaluation of large extracellular vesicles using the attune™ NXT system.
Lugo-Gavidia, Leslie Marisol; Burger, Dylan; Bosio, Erika; Robinson, Sandi; Nolde, Janis; Carnagarin, Revathy; Matthews, Vance B; Schlaich, Markus P.
Afiliação
  • Lugo-Gavidia LM; Dobney Hypertension Centre, Medical School - Royal Perth Hospital Unit, Medical Research Foundation, The University of Western Australia, Perth, Australia.
  • Burger D; Kidney Research Centre, The Ottawa Hospital Research Institute, Department of Cellular and Molecular Medicine, University of Ottawa, Canada.
  • Bosio E; Centre for Clinical Research in Emergency Medicine, Harry Perkins Institute of Medical Research, Perth, Western Australia, Australia.
  • Robinson S; Dobney Hypertension Centre, Medical School - Royal Perth Hospital Unit, Medical Research Foundation, The University of Western Australia, Perth, Australia.
  • Nolde J; Dobney Hypertension Centre, Medical School - Royal Perth Hospital Unit, Medical Research Foundation, The University of Western Australia, Perth, Australia.
  • Carnagarin R; Dobney Hypertension Centre, Medical School - Royal Perth Hospital Unit, Medical Research Foundation, The University of Western Australia, Perth, Australia.
  • Matthews VB; Dobney Hypertension Centre, Medical School - Royal Perth Hospital Unit, Medical Research Foundation, The University of Western Australia, Perth, Australia.
  • Schlaich MP; Dobney Hypertension Centre, Medical School - Royal Perth Hospital Unit, Medical Research Foundation, The University of Western Australia, Perth, Australia; Department of Internal Medicine, Royal Perth Hospital, Perth, Western Australia, Australia; Departments of Cardiology and Nephrology, Royal Pert
J Immunol Methods ; 499: 113170, 2021 12.
Article em En | MEDLINE | ID: mdl-34662560
ABSTRACT
Extracellular vesicles (EVs) represent a promising biomarker in several medical areas. Flow cytometry (FC) is one of the most widely-used methods to characterize EVs, providing quantitative information and determination of EV subtypes. EV evaluation represents a challenge as no standardized methods are available to facilitate assessment across different research centers. This is principally because their size falls below the detection limit of most standard flow cytometers and a thorough optimization process is required to ensure instrument-specific sensitivity. We provide an overview of a standardized method to evaluate large EVs using the Attune™ Nxt Acoustic Focusing Flow Cytometer system (Thermo Fisher Scientific).
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Citometria de Fluxo / Vesículas Extracelulares Limite: Humans Idioma: En Revista: J Immunol Methods Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Austrália
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Citometria de Fluxo / Vesículas Extracelulares Limite: Humans Idioma: En Revista: J Immunol Methods Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Austrália