Identification of miRNAs Bound to an RNA of Interest by MicroRNA Capture Affinity Technology (miR-CATCH).
Methods Mol Biol
; 2404: 207-218, 2022.
Article
em En
| MEDLINE
| ID: mdl-34694611
ABSTRACT
microRNA capture affinity technology (miR-CATCH) uses affinity capture biotinylated antisense oligonucleotides to co-purify a target transcript together with all its endogenously bound miRNAs. The miR-CATCH assay is performed to investigate miRNAs bound to a specific mRNA. This method allows to have a total vision of miRNAs bound not only to the 3'UTR but also to the 5'UTR and Coding Region of target messenger RNAs (mRNAs).
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
MicroRNAs
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Itália