Targeting prostate cancer with Clostridium perfringens enterotoxin functionalized nanoparticles co-encapsulating imaging cargo enhances magnetic resonance imaging specificity.
Nanomedicine
; 40: 102477, 2022 02.
Article
em En
| MEDLINE
| ID: mdl-34740868
ABSTRACT
Magnetic resonance is a key imaging tool for the detection of prostate cancer; however, better tools focusing on cancer specificity are required to distinguish benign from cancerous regions. We found higher expression of claudin-3 (CLDN-3) and -4 (CLDN-4) in higher grade than lower-grade human prostate cancer biopsies (nâ¯=â¯174), leading to the design of functionalized nanoparticles (NPs) with a non-toxic truncated version of the natural ligand Clostridium perfringens enterotoxin (C-CPE) that has a strong binding affinity to Cldn-3 and Cldn-4 receptors. We developed a first-of-its-type, C-CPE-NP-based MRI detection tool in a prostate tumor-bearing mouse model. NPs with an average diameter of 152.9⯱â¯15.7â¯nm (RS1) had a 2-fold enhancement of tumor specificity compared to larger (421.2⯱â¯33.8â¯nm) NPs (RS4). There was a 1.8-fold (Pâ¯<â¯0.01) and 1.6-fold (Pâ¯<â¯0.01) upregulation of the tumor-to-liver signal intensities of C-RS1 and C-RS4 (functionalized NPs) compared to controls, respectively. Also, tumor specificity was 3.1-fold higher (Pâ¯<â¯0.001) when comparing C-RS1 to C-RS4. This detection tool improved tumor localization of contrast-enhanced MRI, supporting potential clinical applicability.
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Bases de dados:
MEDLINE
Assunto principal:
Neoplasias da Próstata
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Nanopartículas
Limite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Nanomedicine
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2022
Tipo de documento:
Article