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A genetically encoded fluorescent biosensor for extracellular L-lactate.
Nasu, Yusuke; Murphy-Royal, Ciaran; Wen, Yurong; Haidey, Jordan N; Molina, Rosana S; Aggarwal, Abhi; Zhang, Shuce; Kamijo, Yuki; Paquet, Marie-Eve; Podgorski, Kaspar; Drobizhev, Mikhail; Bains, Jaideep S; Lemieux, M Joanne; Gordon, Grant R; Campbell, Robert E.
Afiliação
  • Nasu Y; Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.
  • Murphy-Royal C; Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, T2N 4N1, Canada.
  • Wen Y; Centre Hospitalier de l'Université de Montréal, Department of Neuroscience, Faculty of Medicine, University of Montreal, Montreal, QC, H2X 0A9, Canada.
  • Haidey JN; Department of Biochemistry, University of Alberta, Edmonton, AB, T6G 2H7, Canada.
  • Molina RS; Department of Talent Highland, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China.
  • Aggarwal A; Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, T2N 4N1, Canada.
  • Zhang S; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, 59717, USA.
  • Kamijo Y; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA.
  • Paquet ME; Department of Chemistry, University of Alberta, Edmonton, AB, T6G 2G2, Canada.
  • Podgorski K; Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.
  • Drobizhev M; CERVO Brain Research Center, Laval University, Québec, QC, G1E 1T2, Canada.
  • Bains JS; Department of Biochemistry, Microbiology and Bioinformatics, Laval University, Québec, QC, G1J 2G3, Canada.
  • Lemieux MJ; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA.
  • Gordon GR; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, 59717, USA.
  • Campbell RE; Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, T2N 4N1, Canada.
Nat Commun ; 12(1): 7058, 2021 12 06.
Article em En | MEDLINE | ID: mdl-34873165
L-Lactate, traditionally considered a metabolic waste product, is increasingly recognized as an important intercellular energy currency in mammals. To enable investigations of the emerging roles of intercellular shuttling of L-lactate, we now report an intensiometric green fluorescent genetically encoded biosensor for extracellular L-lactate. This biosensor, designated eLACCO1.1, enables cellular resolution imaging of extracellular L-lactate in cultured mammalian cells and brain tissue.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Proteínas Recombinantes de Fusão / Técnicas Biossensoriais / Ácido Láctico / Proteínas Periplásmicas / Proteínas de Fluorescência Verde Limite: Humans Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Japão
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Proteínas Recombinantes de Fusão / Técnicas Biossensoriais / Ácido Láctico / Proteínas Periplásmicas / Proteínas de Fluorescência Verde Limite: Humans Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Japão