Development of Three-Stage Bioaerosol Sampler for Size-Selective Sampling.
J Biomech Eng
; 144(7)2022 07 01.
Article
em En
| MEDLINE
| ID: mdl-35013744
ABSTRACT
In this study, a three-stage bio-aerosol sampler with a sampling flow rate of 170 L/min was designed and fabricated for sampling the bio-aerosols released during human breathing and coughing, and its performance was evaluated. The sampler was constructed using a cyclone separator with a cutoff size of 2.5 µm as a preseparator, a multinozzle virtual impactor with a cutoff size of 0.34 µm as an aerosol concentrator, and a Bio-Sampler, which is a commercial product, for collecting bio-aerosols in a collection fluid. The collection efficiency of the sampler was evaluated through simulations and experiments. Only particles with sizes of 0.1-4 µm were selectively collected in the collection fluid. Bacteriophage bio-aerosols were sampled using the developed sampler and ACD-200 Bobcat sampler, which is a commercial product. The amounts of collected bacteriophages were compared using the polymerase chain reaction (PCR) technique. The sampling performance of the developed sampler was similar to that of the ACD-200 Bobcat sampler. Moreover, the developed sampler showed its ability to sample bio-aerosols of a specific size range and collect them directly in a collection fluid for the PCR analysis. Therefore, the developed sampler is expected to be useful for indoor environmental monitoring by effectively sampling the bio-aerosols released indoors during human breathing and coughing.
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Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Manejo de Espécimes
/
Monitoramento Ambiental
Limite:
Humans
Idioma:
En
Revista:
J Biomech Eng
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Coréia do Sul