Genomic surveillance of SARS-CoV-2 Spike gene by sanger sequencing.
PLoS One
; 17(1): e0262170, 2022.
Article
em En
| MEDLINE
| ID: mdl-35051202
ABSTRACT
The SARS-CoV-2 responsible for the ongoing COVID pandemic reveals particular evolutionary dynamics and an extensive polymorphism, mainly in Spike gene. Monitoring the S gene mutations is crucial for successful controlling measures and detecting variants that can evade vaccine immunity. Even after the costs reduction resulting from the pandemic, the new generation sequencing methodologies remain unavailable to a large number of scientific groups. Therefore, to support the urgent surveillance of SARS-CoV-2 S gene, this work describes a new feasible protocol for complete nucleotide sequencing of the S gene using the Sanger technique. Such a methodology could be easily adopted by any laboratory with experience in sequencing, adding to effective surveillance of SARS-CoV-2 spreading and evolution.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Análise de Sequência de RNA
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Pandemias
/
Glicoproteína da Espícula de Coronavírus
/
Genes Virais
/
Teste de Ácido Nucleico para COVID-19
/
SARS-CoV-2
/
COVID-19
Tipo de estudo:
Screening_studies
Limite:
Humans
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
PLoS One
Assunto da revista:
CIENCIA
/
MEDICINA
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Brasil