Molecular mapping for fruit-related traits, and joint identification of candidate genes and selective sweeps for seed size in melon.
Genomics
; 114(2): 110306, 2022 03.
Article
em En
| MEDLINE
| ID: mdl-35131474
ABSTRACT
Melon is a popular fruit vegetable crop worldwide with diverse morphological variation. We report a high-density genetic map of melon and nine major QTLs with physical region ranging from 43.47 kb to 1.89 Mb. Importantly, two seed-related trait QTLs were repeatedly detected in two environments, and the mapping region was narrowed to 522 kb according to a regional linkage analysis. A total of 40 annotated genes were screened for nonsynonymous variations, of which EVM0009818, involved in cytokinin-activated signaling, was differentially expressed in the young fruits of parents based on RNA-seq. Selective sweep analysis identified 152 sweep signals for seed size, including the two seed-related QTLs and nine homologs that have been verified to regulate seed size in Arabidopsis or rice. This work illustrates the power of a joint analysis combining resequencing-based genetic map for QTL mapping and a combination of KASP genotyping and RNA-seq analysis to facilitate QTL fine mapping.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Cucurbitaceae
/
Frutas
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
Genomics
Assunto da revista:
GENETICA
Ano de publicação:
2022
Tipo de documento:
Article