Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing.

Ge, Yang; van Roon, Lieke; Chen, H Sophia; Methorst, Ruben; Paton, Martin; DeRuiter, Marco C; Kielbasa, Szymon M; Jongbloed, Monique R M

Ge, Yang; van Roon, Lieke; Chen, H Sophia; Methorst, Ruben; Paton, Martin; DeRuiter, Marco C; Kielbasa, Szymon M; Jongbloed, Monique R M.

Afiliação

Ge Y; Department of Anatomy & Embryology, Leiden University Medical Center; Department of Cardiology, Leiden University Medical Center.
van Roon L; Department of Anatomy & Embryology, Leiden University Medical Center.
Chen HS; Department of Anatomy & Embryology, Leiden University Medical Center; Department of Cardiology, Leiden University Medical Center.
Methorst R; Department of Anatomy & Embryology, Leiden University Medical Center.
Paton M; Department of Cardiology, Leiden University Medical Center.
DeRuiter MC; Department of Anatomy & Embryology, Leiden University Medical Center.
Kielbasa SM; Department of Medical Statistics and Bioinformatics, Leiden University Medical Center.
Jongbloed MRM; Department of Anatomy & Embryology, Leiden University Medical Center; Department of Cardiology, Leiden University Medical Center; m.r.m.jongbloed@lumc.nl.

J Vis Exp ; (181)2022 03 23.

Article em En | MEDLINE | ID: mdl-35404341

Assuntos

Células Endoteliais; Gânglios Simpáticos; Animais; Sistema Nervoso Autônomo; Camundongos; RNA Nuclear Pequeno; Análise de Sequência de RNA/métodos

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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Células Endoteliais / Gânglios Simpáticos Tipo de estudo: Guideline Limite: Animals Idioma: En Revista: J Vis Exp Ano de publicação: 2022 Tipo de documento: Article

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