The perinuclear theca protein Calicin helps shape the sperm head and maintain the nuclear structure in mice.
Cell Rep
; 40(1): 111049, 2022 07 05.
Article
em En
| MEDLINE
| ID: mdl-35793634
ABSTRACT
The perinuclear theca (PT) is a cytoskeletal element encapsulating the sperm nucleus; however, our understanding of the physiological roles of PT in sperm is very limited. We show that Calicin interacts with itself and many other PT components, indicating it may serve as an organizing center of the PT assembly. Calicin is detectable first when surrounding the acrosome, then detected around the entire nucleus, and finally translocated to the postacrosomal region of spermatid heads. Intriguingly, loss of Calicin specifically causes surface subsidence of sperm heads in the nuclear condensation stage. Calicin interacts with inner acrosomal membrane (IAM) protein Spaca1 and nuclear envelope (NE) components to form an "IAM-PT-NE" structure. Intriguingly, Ccin-knockout sperm also exhibit DNA damage and failure of fertilization. Our study provides solid animal evidence to suggest that the PT encapsulating sperm nucleus helps shape the sperm head and maintain the nuclear structure.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Sêmen
/
Cabeça do Espermatozoide
/
Proteínas do Citoesqueleto
Limite:
Animals
Idioma:
En
Revista:
Cell Rep
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
China