Split luciferase-based estimation of cytosolic cargo concentration delivered intracellularly via attenuated cationic amphiphilic lytic peptides.

Okano, Syusuke; Kawaguchi, Yoshimasa; Kawano, Kenichi; Hirose, Hisaaki; Imanishi, Miki; Futaki, Shiroh

Okano, Syusuke; Kawaguchi, Yoshimasa; Kawano, Kenichi; Hirose, Hisaaki; Imanishi, Miki; Futaki, Shiroh.

Afiliação

Okano S; Institute for Chemical Research Kyoto University, Uji, Kyoto 611-0011, Japan.
Kawaguchi Y; Institute for Chemical Research Kyoto University, Uji, Kyoto 611-0011, Japan.
Kawano K; Institute for Chemical Research Kyoto University, Uji, Kyoto 611-0011, Japan.
Hirose H; Institute for Chemical Research Kyoto University, Uji, Kyoto 611-0011, Japan.
Imanishi M; Institute for Chemical Research Kyoto University, Uji, Kyoto 611-0011, Japan.
Futaki S; Institute for Chemical Research Kyoto University, Uji, Kyoto 611-0011, Japan. Electronic address: futaki@scl.kyoto-u.ac.jp.

Bioorg Med Chem Lett ; 72: 128875, 2022 09 15.

Article em En | MEDLINE | ID: mdl-35798239

Assuntos

Endossomos; Peptídeos; Cátions/metabolismo; Citosol/metabolismo; Endossomos/metabolismo; Luciferases/metabolismo; Peptídeos/química

Palavras-chave

Attenuated cationic amphiphilic lytic (ACAL) peptide; Cytosolic concentration; Endosomal release; Intracellular delivery; Split-NanoLuc luciferase assay

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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Peptídeos / Endossomos Idioma: En Revista: Bioorg Med Chem Lett Assunto da revista: BIOQUIMICA / QUIMICA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Japão

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